Figure 2
From: Activation of adherent vascular neutrophils in the lung during acute endotoxemia
Effects of acute endotoxemia on DNA fragmentation, Annexin V binding and Mcl-1 expression in adherent vascular lung neutrophils. Cells were isolated from control animals or 2–48 hours after endotoxin administration. Upper Panel: Cytoplasmic DNA was extracted, analyzed on 1.2 % agarose gels and visualized after staining with ethidium bromide. Middle Panel: Cells were analyzed for Annexin V binding by flow cytometry. Data are expressed as % Annexin V positive cells. Lower Panel: Cytoplasmic extracts were prepared and 10 μg protein/well analyzed for Mcl-1 protein by western blotting. Positive control (RAW 264.7 mouse macrophage lysates) is indicated by the + symbol. One representative gel of three separate experiments is shown. CTL = control