Figure 2
From: Binding of protegrin-1 to Pseudomonas aeruginosa and Burkholderia cepacia
Quantitative aspects of PG-1 mediated killing measured by two-stage radial diffusion assays. The left ordinate scale shows the net area of underlay gel totally cleared of viable bacteria using the equation (π d2-9)÷ 4, where d is the diameter of the cleared zone including the well diameter and 9 is subtracted to account for the area of the well, which is devoid of bacteria. The right ordinate scale shows the volume cleared (area cleared times the height of the underlay gel, 1.2 mm). All data points are means (n = 3). Note that Y is a linear function of X for these peptide concentrations. Area (A) and volume (V) are related by the equation: A (mm2) = V (μl)/1.2 mm.