Figure 2

Effects of prolonged hypoxia on mRNA and protein levels of Kv channel α- and β-subunits, in PASMCs and mesenteric artery smooth muscle cells (MASMCs). (a) Polymerase chain reaction-amplified products for Kv1.2 (295 bp) and β-actin (244 bp) displayed in agarose gel. PASMCs were incubated in normoxia (Nor) and hypoxia (25-35 mmHg) for 24 h (H24), 36 h (H36), 48 h (H48), and 60 h (H60). (b) mRNA levels (mean ± standard error) of Kv1.2, Kv1.4, Kv1.5, and Kvβ1.1 in PASMCs, normalized to the amount of β-actin. (c) Western blot analysis of Kv1.2 channel proteins from PASMCs incubated in normoxia (Nor) and hypoxia (25-35 mmHg) for 48 h (H48), 60 h (H60), and 72 h (H72). (d) mRNA levels (mean± standard error), normalized to the respective amounts of β-actin, in PASMCs (left panel) and MASMCs (right panel) before (Nor) and after (Hyp) treatment with hypoxia. ^** P < 0.01 versus Nor. M, 100-bp DNA ladder. Modified with permission from Wang et al [25**].