Figure 2

Acapsular C. neoformans binds and is internalized by BEAS-2B cells. Light microscopy of differentially stained BEAS-2B cells that were stimulated for 24 h with acapsular C. neoformans cultured at RT (A), 37°C (E), or 37°C followed by trypsinization (I). Flow cytometric analysis of BEAS-2B cells that were stimulated for 3 h with unlabeled (B and F) or FITC-labeled (C and G) acapsular C. neoformans grown at RT or 37°C respectively, and then quenched with trypan blue (D and H). Data are representative of three independent experiments.