Expression of IL-13 receptor subunits in cultured HASM. The following primary antibodies were used: anti-IL-13Rα1 mAb, anti-IL-13Rα2 mAb and anti-IL-4Rα mAb. These primary antibodies were detected with Cy2-conjugated goat anti-mouse IgG (green). Slides were counterstained with nuclei stain DAPI (blue). As control, cells were processed in the absence of the primary antibody and/or the secondary antibody. mRNA transcripts for all subunits in HASM cells obtained from two different subjects are also shown: lane 1: IL-13Rα1; lane 2: IL-13Rα2; lane 3: IL-4Rα.