Table 1 RT-PCR primers, conditions and products
From: Attenuation of acute lung inflammation induced by cigarette smoke in CXCR3 knockout mice
RT-PCR Genes | S/AS | Primer sequence (5' to 3') | Tm(°C) | Product (bp) |
|---|---|---|---|---|
CXCL9 | S | CTTGGGCATCATCTTCCT G | 55 | 352 |
AS | TGAACGACGACGACTTTGG | |||
CXCL10 | S | GTCATTTTCTGCCTCATCC | 55 | 273 |
AS | GAGCCCTTTTAGACCTTTT | |||
CXCL11 | S | CTGCTCAAGGCTTCCTTATGTT | 55 | 166 |
AS | CCTTTGTCGTTTATGAGCCTTC | |||
IFNγ | S | CATCTTGGCTTTGCAGCTCTT | 55 | 363 |
AS | CTGGACCTGTGGGTTGTTGA | |||
Granzyme-A | S | GAAACCAGGAACCAGATGC | 55 | 390 |
AS | GTGACAGGGATGGAGTGAA | |||
Granzyme-B | S | CCCTCTGCCTTCTTCCTC | 55 | 344 |
AS | CTGGGTCTTCTCCTGTTCTT | |||
Perforin | S | ATGGCACGCACTTTATCAC | 55 | 413 |
AS | CTTCGGGTTCTGTTCTTCC | |||
β-actin | S | CTTCCTTAATGTCACGCACGATTTC | 55 | 541 |
AS | GTGGGGCGGCCCAGGCACCA |