Oxidized SP-A content in BAL of FA-exposed and ozone-exposed WT male and female mice. Experimental design was as described in Figure 5. FA-exposed groups are shown with open bars, and ozone-exposed groups – with solid bars. Estimation of total SP-A concentration (7A) and oxidized SP-A dimers (7B) in BAL was performed as described in Methods. Samples used for the estimation of SP-A (7A) and oxidized SP-A (7B) were from same aliquots. For SP-A quantification, SP-A bands corresponding to monomer, dimer, or monomer + dimer (total) SP-A were analyzed. Data for BAL samples in 7A and 7B were from two different blots. To facilitate analysis, data from each mouse BAL sample were normalized to the average of the positive controls (hSP-A) from the two blots, according to the following formula: OD × mm2 of mouse SP-A sample/OD × mm2 of hSP-A times 100%. For oxidized mouse SP-A dimer estimation, oxidized hSP-A dimer was used as a denominator. The oxyblot from the two experiments (# 1 and # 2) depicting SP-A dimer oxidation is shown in 7C. Data were considered to be significant if p < 0.05 with a t-test. Significant differences are shown with lines above the respective bars. M, monomer SP-A; D, dimer SP-A; T, total (monomer + dimer) SP-A.