Total protein oxidation in BAL of FA-exposed and ozone-exposed WT male and female mice. Experimental design was as described in Figure 5. After the mouse lungs were lavaged, BAL was centrifuged, and the supernatant was used for the estimation of total protein concentration in BAL (6A) and for total oxidized protein analysis (6B) as described in Methods. An aliquot of the same supernatant analyzed in Figure 5 was also used here. The OD × mm2 values were calculated for each sample. FA-exposed groups are shown with opened bars, and ozone-exposed groups – with solid bars. Significance between different groups of mice is shown with connecting lines above the respective bars. Statistical analysis was performed with a t-test and the differences were considered as significant if p < 0.05.