Differential cell counts in BAL of FA-exposed and ozone-exposed WT male and female mice. Experimental design was as described for in vivo phagocytosis (Figure 4), except that PBS was injected into mice instead of bacteria (see Methods). The percent of polymorphonuclear neutrophils (PMNs), lymphocytes, and macrophages/monocytes was calculated and compared between ozone-exposed and FA-exposed mice and between males and females. The percent of lymphocytes did not differ significantly among different groups. The number of mice in this analysis is shown in the Figure. The number of independent experiments was 4, where each experiment initially involved 6 mice (3 FA-exposed mice and 3 ozone-exposed). Significant differences are shown with lines above the respective bars. Statistical analysis was performed with a t-test and the differences were considered significant if p < 0.05.