In vivophagocytosis ofK. pneumoniaebacteria by alveolar macrophages isolated from SP-A (-/-) mice exposed to ozone or FA. Experimental design is described in Methods. Absolute data were used for this analysis. Data from filtered air and ozone-exposed WT mice (on the left broken lines) are from Mikerov et al.  and are presented here for comparison purposes. Reference (dotted lines) in the figure are shown for convenience to demonstrate the similarity of the phagocytic indices of ozone-exposed WT mice and filtered air-exposed SP-A (-/-) mice. Differences were considered significant if p < 0.05 with a t-test. *Significant differences between FA exposed and ozone exposed mice of the same sex; **significant differences between males and females after ozone exposure; §significant differences between SP-A (-/-) and WT mice of the same sex and after the same treatment (i.e. ozone-exposed or FA-exposed). The number of independent experiments for female and male SP-A (-/-) mice was 5 and 4, respectively. The number (n) of mice in each group that survived after the procedure is shown.