IL-8 induced phosphorylation of MLC
from CF and control cells. Panel A shows representative blots of myosin light chain (MLC20) phosphorylation from CF and control cells. Bands correspond to baseline and IL-8 stimulation at 1 and 5 minutes. Thiophosphorylated myosin from chicken gizzard was used as a positive control (+ve con). Panel B shows the average increase in MLC20 phosphorylation (expressed as fold difference from baseline) in CF and control cells. The MLC20 phosphorylation from CF cells was significantly different from control cells at 1 minute after treatment with IL-8.