Effects of IL-8 on [Ca2+]
in CF and control cells. Cultured ASM cells from CF and control subjects were stimulated with IL-8 (100 nM). Illustrative examples of responses of a control cell and a cell from a CF-affected subject are shown in panel 1. The left hand arrow indicates the addition of IL-8 to the medium and the right hand arrow represents the addition of histamine (1 μM) to serve as a positive control. The resting [Ca2+]i (R) and the peak [Ca2+]i induced by IL-8 (IL-8) from the control (open bars) and the CF group (hatched bars) are shown. (n = 48 cells recorded on 6 different slides from 4 individuals in each group).