Contraction of CF and control ASM cells treated with IL-8. Panels A and B show the images recorded before and 10 minutes after the addition of IL-8 (100 nM) in CF cells. The cells that are clearly visualized are live cells and the indistinct cells are the background of alcohol-fixed cells that serve as a substratum. Arrows indicate the contracted cells. Panel C and D show the images of control cells before and after the addition of IL-8. Panel E represents the % decrease in the CF and non-CF cell lengths (C) following IL-8 or PBS treatments. Cumulative frequency distributions are shown and the distributions were compared statistically using the Kolmogorov-Smirnoff test. The IL-8 treated CF cells shortened to a significantly greater degree than the non-CF cells (P < 0.05). 40 CF cells and 36 control cells from four different individuals per group were measured. The values are expressed in % decrease in the length of the cell following IL-8 stimulation.