Figure 7

Flash photolysis of iso-Ins(1,4,5)P3/PM induced release from internal stores in human tracheal gland cells. A Typical traces of Ca²⁺ mobilization in CF-KM4 cells during 5 min stimulation by 100 μM histamine or 10 mM caffeine in absence of extracellular Ca²⁺. B The CF-KM4 cells were loaded with fluo-4 and without iso-Ins(1,4,5)P3/PM and stimulated by UV light. C Traces show average normalized fluo4 fluorescence recordings in uncorrected or corrected CF-KM4 (incubated 2 h at 37°C with 100 μM miglustat) and in MM39 cells in absence of extracellular Ca²⁺. These cells were preincubated during 10 min in presence or not of 100 μM 2-APB. D Histogram of the normalized area under curve of intensity profile of Ca²⁺ response in various experimental conditions as indicated. These cells were incubated 2 h at 37°C with 100 μM miglustat (CF-KM4 and CF-KM4 reverted cells). Results are presented as mean ± SEM and the number of experiments is noted on each bar graph. * P < 0.05; ** P < 0.01*** P < 0.001; ns, non significant difference.