Figure 4

Modification of local stimulation of caged Ca²⁺in corrected F508del-CFTR CF15 cells. A Relative mRNA expression level of IP₃R-1, IP₃R-2, and IP₃R-3 in different conditions compared to βActin mRNA expression. B Example of line-scan images acquired at 2 ms per line and 0.21 μm per pixel in CF15 cells treated (27°C, miglustat, NB-DGJ and uncorrected at 37°C in absence of extracellular Ca²⁺). C Average of the line-scan images in B expressed as normalized fluorescence in absence of extracellular Ca²⁺. D Histograms showing the amplitude of IP₃Rs Ca²⁺ response in various experimental conditions as indicated. E Mean normalized area in each experimental treatment in absence or presence of 10 μM CsA. Sets of data were compared to the control CF15. Results are presented as mean ± SEM and the number of experiments is noted on each bar graph. ** P < 0.01, *** P < 0.001; ns, non significant difference.