Figure 3

Pharmacology of IP ₃ R response of local uncaging of caged Ca²⁺in CF15 cells in absence of extracellular Ca²⁺. A Example of line-scan images acquired at 2 ms per line and 0.21 μm per pixel in CF15 cells untreated at 37°C in presence or not of 100 μM 2-APB, 100 μM decavanadate, 20 mM caffeine or 10 μM cyclosporine A (all were preincubated during 10 min) and after 2 h incubation with 10 μM thapsigargin (TG). B Average of the line-scan images in A expressed as normalized fluorescence in each conditions C Mean normalized area measured from XT images in each experimental condition. The dash line represents the response induced by the flash only, after complete ER Ca²⁺ store depletion. Results are presented as mean ± SEM and the number of experiments is noted on each bar graph. * P < 0.05; ** P < 0.01*** P < 0.001; ns, non significant difference.