Characterization of GM-MØ. CD14 (A), HLA-DR (B) and HLA-DQ (C) expression were evaluated by flow cytometry. Solid lines represent the fluorescence of stained cells, while dashed lines represent the results from control antibodies. Data are representative of experiments performed on cells from three donors. Cytocentrifuge preparations illustrating monocyte and macrophage morphology before (D) and after (E) maturation with GM-CSF were captured at equal magnification (200×).