Figure 4

Effect of bleomycin instillation on leukocyte subset recruitment into the lungs of mice. Cathepsin K transgenic mice (black bars) and wild-type mice (white bars) were administered either saline or bleomycin for 1, 2, 3, and 4 weeks. Subsequently, mice were euthanized and lungs were lavaged as described in Materials and Methods. Total BAL fluid cell numbers (A), alveolar macrophages (B), exudate macrophages (C), neutrophils (D) and lymphocytes (E) were ascertained from Pappenheim-stained cytospin preparations of BAL fluid. The data are shown as mean ± SD of at least n = 5 mice per group and time point. No statistical significances were noted between wild-type and Cathepsin K transgenic mice.