Figure 5
From: Cigarette smoke worsens lung inflammation and impairs resolution of influenza infection in mice
TIMP-1 protein in BALF. TIMP-1 was quantitated by ELISA (a) and real time PCR of lung mRNA (b). Clear boxes denote mice not infected with influenza and dark boxes denote mice infected with influenza. BALFs of the 8 mice in each group were pooled and used in duplicate ELISAs for TIMP-1. Legend in (b): â– = influenza alone mice, â–² = smoke alone and - â—† - = smoke and influenza mice. Tissue pieces of equivalent size from lungs of the 4 mice in each treatment group were pooled prior to RNA extraction, and the cDNA was used in triplicate reactions. Fold changes are expressed relative to mRNA levels in lungs of no treatment mice.