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Figure 4 | Respiratory Research

Figure 4

From: Cigarette smoke worsens lung inflammation and impairs resolution of influenza infection in mice

Figure 4

Lung protease activity and gene expression. In bar graphs clear boxes denote mice not infected with influenza and dark boxes denote mice infected with influenza. Panels a-h show protease activity in BALF. (a) Zymography of gelatinase activity at d3, (b) zymography of caseinolytic activity at d3 (the molecular mass of protein standards in kDa is indicated on the left side), (c) densitometry of MMP-9, (d) densitometry of MMP-2, (e) densitometry of 90 kDa caseinolytic protease, (f) densitometry of 60 kDa caseinolytic protease, (g) net gelatinase activity and (h) net serine protease activity in BALF, determined by fluorometric and colorimetric assays (respectively) in 96 well plates. BALFs of the 8 mice in each group were pooled for all of these assays. For the assays in panels g and h, this graph is representative of two assays run with the same samples, and every sample was run in triplicate. Panel i shows quantitation of relative mRNA units of proteases in mouse lung. Legend: â–  = influenza alone mice, â–² = smoke alone and - â—† - = smoke and influenza mice. Tissue pieces of equivalent size from lungs of the 4 mice in each treatment group were pooled prior to RNA extraction, and the cDNA was used in triplicate reactions. Fold changes are expressed relative to mRNA levels in lungs of no treatment mice.

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