Figure 4
Co-localisation of TNFα with macrophages in the lung parenchyma. Lung tissue was obtained from a 65 year-old female smoker (GOLD 1) stimulated with LPS for 1 hour. The tissue was then embedded and sequential sections of the lung parenchyma stained with monoclonal antibodies for TNFα (figure 4A and 4C) and CD68 (4B) and mast cell tryptase (4D). Staining specificity was determined by IgG1 isotype antibody controls 1:200 (4E) and 1:1000 (4F) for CD68 and mast cell tryptase respectively. Bars represents 10 μm, positive cells are stained red.