Figure 3
Effect of LTD 4 , LTE 4 and TGF-β 1 on the expression of laminin (Ln) β2 chain in BEAS-2B human bronchial epithelial cells. Western blot analysis revealed increased expression of Ln β2 chain in the cell culture medium in response to stimulation with LTD4 (A), but not with LTE4 (B) for 48 h. RT-PCR analysis detected increased expression of Ln β2 chain mRNA in response to 100 nM LTD4 (D), but no effect with either LTE4 (D) or TGF-β1 (E). The increase in response to LTD4 was concentration-dependent. The data were quantified by densitometry and are expressed as fold increases above non-stimulated cells (mean ± SEM) (n = 12). *p < 0.05 and ***p < 0.001 vs. non-stimulated cells. C: Immunoperoxidase immunocytochemistry for Ln β2 chain showing no definite expression of Ln β2 chain by non-stimulated control cells with a slight increase after treatment with both LTD4 and LTE4. Images are reproduced from a single experiment, but are representative findings of multiple staining experiments.