Figure 2
Effect of LTD 4 , LTE 4 , and TGF-β 1 on the expression of tenascin (Tn) in BEAS-2B human bronchial epithelial cells. RT-PCR analysis detected increased expression of Tn mRNA in response to stimulation with LTD4 and LTE4 (A), TGF-β1 (C), and LTD4 combined with TGF-β1 (E) for 48 h. B: Increased expression of Tn was detected by Western blot analysis in cell lysates after stimulation with TGF-β1 (B) for 48 h. The data were quantified by densitometry and are expressed as fold increases above non-stimulated cells (mean ± SEM) (n = 12). D: Augmented expression of Tn protein was detected by flow cytometry in response to LTD4 combined with TGF-β1, compared with the effect of LTD4 alone. Difference from isotype control mean fluorescence intensity (MFI) is presented (mean ± SEM). Presence of 5 mM L-cysteine in the culture media did not affect the expression levels of Tn (D, E). *p < 0.05, **p < 0.01, and ***p < 0.001 vs. non-stimulated cells. NS – non-significant.