Figure 3
AMCase expression in BALF. Western blot analysis and densitometry of BALF demonstrated that AMCase expression parallels general chitinase activity. CA-IT, INF-IT, HK-IT and PBS-IT refer to rats intratracheally inoculated with C. albicans, C. neoformans, heat-killed C. neoformans and PBS respectively (each lane = pooled sample of 6 animals). For densitometry analyses, readings were normalized to GAPDH signal prior to comparison with values obtained from PBS-inoculated animals.