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Table 1 Sequences of primers used to amplify sodAa

From: Superoxide dismutase A antigens derived from molecular analysis of sarcoidosis granulomas elicit systemic Th-1 immune responses

Name

 

Primer sequence

Amplicon length (bp)

Nucleotide (ORF)/amino acid position

Region 1

Fb

5' CCGTGGCCGAATACACCTT 3'

127

-167

 

R

5' CATTGGCGCCCTTTACGTA 3'

  

Region 2

F

5' GAGCTTCACCACAGCAAGCA 3'

127

76–202/26–67

 

R

5' AAGCTAGATTCTTTTCGTTCAGCAA 3'

  

Region 3

F

5' CGTCGCCAAACTCGAAGAG 3'

151

129–279/44–93

 

R

5' GCCGGTGGGCTTGTCA 3'

  

Region 4

F

5' GCCACGTCAATCACACCATCT 3'

232

215–446/73–149

 

R

5' AAGTTCGTCTGGTGGTCGTAAAC 3'

  

Region 5

F

5' GGGACACACTCGGCAACAA 3'

158

389–546/131–182

 

R

5' CAAAACGCCTTGGCAAAGTC 3'

  

Region 6

F

5' TTTACGACCACCAGACGAACTTC 3'

161

425–585/150–195

 

R

5' CGCATACCGTGACTGCACAT 3'

  
  1. aBased on M. tuberculosis H37Rv sodA sequence (GenBank no. AF061030)
  2. bF – forward, R – reverse