Localization of the β
AR in Primary Cultures of Rat Airway Smooth Muscle and Rat Airway Epithelial Cells. Primary cultures of rat ASM cells were derived from tracheal explants. Cells were fixed and double-labeled with β2AR (Ab-13989) (B) and anti-smooth muscle α-actin antibodies (A). C – merged image. Airway epithelial cells were harvested from rat lungs, fixed and double-labeled with β2AR (Ab-13989, E and Ab-sc570, H) and E-cadherin antibodies (D, G, and J). Ab-13989 and Ab-sc570 demonstrated a similar pattern of staining in primary cultures (E and H). Preincubation of Ab-sc570 with neutralizing peptide (sc570p) abrogated the staining (K). Panels F, I, L are merged images.