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Figure 3 | Respiratory Research

Figure 3

From: Characterization of a panel of six β2-adrenergic receptor antibodies by indirect immunofluorescence microscopy

Figure 3

Ab-13989 Specifically Recognizes Human and Rat β 2 AR in HEK 293 Cells. HEK 293 cells stably expressing human β2AR (A, B) and HEK 293 cells transiently expressing rat β2AR (C, D) were either untreated (A, C) or treated (B, D) with isoproterenol for 4.5 h in parallel, fixed and processed for microscopy using a LSM510 confocal microscope. HEK 293 cell lines expressing different levels of human β2AR were processed and analyzed by wide field (E) and confocal (F) microscopy to establish the range over which receptor number and fluorescence signal intensity was linear.

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