Treatments
|
TLR2 (MFIr)
|
TLR4 (MFIr)
|
---|
control
|
1.34 ± 0.1
|
1.81 ± 0.1
|
E. coli
|
1.4 ± 0.03
|
1.66 ± 0.1
|
S. aureus
|
1.3 ± 0.05
|
1.59 ± 0.04
|
- Flow-cytometric analysis of cell surface TLR2 and TLR4 protein in human ASMC. Cells were treated with E. Coli or S. Aureus (108 CFU/ml) for 48 hrs. Data is expressed as ratio of mean fluorescence intensity (MFIr) of cells stained with specific TLR2 or TLR4 antibodies and cells stained with the relevant isotype control antibodies. The data is the mean ± SEM of n = 3–4 ASMC donors.