Treatments | Concentrations | Mean ± s.e.m |
---|
control | 0 | 248.3 ± 76.44 |
S. aureus (CFU ml-1) | 106
| 328.8 ± 132.1 |
| 107
| 170.0 ± 56.09 |
| 108
| 2564 ± 1062 |
PAM 3 (ng ml-1) | 3 | 405.3 ± 103.0 |
| 30 | 2108 ± 531.5 |
| 300 | 7994 ± 2178.0 |
FSL1 (ng ml-1) | 10 | 370.6 ± 144.4 |
| 100 | 509.0 ± 214.4 |
| 1000 | 3792 ± 1379 |
MDPLys18 (10 nM) | 10 | 218.1 ± 95.11 |
| 100 | 444.1 ± 143.1 |
- Effect of Gram-positive bacteria and associated PAMPs on CXCL-8 release from human ASMCs over 48 h. ASMCs were treated either Gram-positive bacteria S. aureus, (106–108 CFU ml-1), Pam3CSK4 (3 – 300 ng ml-1), FSL-1, (10 – 1000 ng ml-1) or MDPLys18 (10 nM and 100 nM). The data represent the mean ± SEM of triplicate of three different donors.