Treatments | Concentrations | Mean ± s.e.m |
---|
control | 0 | 298 ± 71.17 |
E. coli (CFU ml-1) | 106
| 4566 ± 1218 |
| 107
| 7060 ± 1866 |
| 108
| 17130 ± 5373 |
LPS (μg ml-1) | 0.01 | 2500 ± 739.5 |
| 0.1 | 4392 ± 1131 |
| 1 | 4217 ± 1050 |
FK565 (10 nM) | 10 | 177.3 ± 88.16 |
| 100 | 150.8 ± 67.31 |
- Effect of Gram-negative bacteria and associated PAMPs on CXCL-8 release from human ASMCs over 48 h. ASMCs were treated with either bacteria Gram-negative E. coli, (106–108 CFU ml-1), LPS, (0.01–1 μg ml-1) or FK565, (10 nM and 100 nM). The data represent the mean ± SEM from triplicate of three different donors.