Figure 7

(R+S) and (R)-albuterol decrease NF-κB activity. A NF-κB luciferase reporter construct (pGL2) was cotransfected with a β-galactosidase (pGK) reporter construct by electroporation into T (EL4) cells. After transfection, (R)-albuterol (R), (S)-albuterol (S), or racemic albuterol (R+ S) were added to cells pre-activated with (ConA, 5 μg/ml) and (PMA, 100 ng/ml) (CP) and then treated with (R)-, (S)-, or (R+S)-albuterol (Fig. 6). Relative light units (RLU) were normalized to β-gal (pGK) coreporter activity. Fold induction is the ratio of RLU of stimulated to unstimulated sample. Data are shown as mean ± SEM (n = 3).