Figure 1
From: IL-13 induces a bronchial epithelial phenotype that is profibrotic
Protocol for IL-13 treatment and withdrawal. NHBE cells are seeded on Transwells® as described in the Materials and Methods, treatment with IL-13 followed by its withdrawal is carried out as shown. (A) For the control case, the NHBE are cultured in 50:50 epithelial media without any IL-13 all throughout and co-cultured with NHLF embedded in rat tail collagen gel from days 22 to day 25 and day 28 to day 31. (B) NHBE are treated for 14 days from day 7 to day 21 with varying IL-13 concentrations (0.1, 1, 10 ng/ml), then the IL-13 media is withdrawn and replaced with 50:50 epithelial media for 1 day from day 21 to day 22. On day 22 the NHBE are co-cultured with NHLF embedded in a rat-tail collagen gel for a period of 3 days till day 25. (C) The NHBE are treated for 14 days from day 7 to day 21 with varying IL-13 concentrations (0.1, 1, 10 ng/ml), then the IL-13 media is withdrawn and replaced with 50:50 media for a period of 7 days from day 21 to day 28. On day 28 the NHBE are co-cultured with NHLF embedded in a rat-tail collagen gel for 3 days till day 31.