Figure 6
From: Regulation of the cd38 promoter in human airway smooth muscle cells by TNF-α and dexamethasone
Binding of recombinant glucocorticoid receptor (GR) to consensus GRE sequences. Binding of recombinant GR to labeled consensus GRE sequence (lane 2 and indicated by horizontal arrow), competition for binding with cd38 putative GRE sequences (labeled GRE1 to GRE4, lanes 7–10), and gel supershift with anti-GR antibodies (Anti-GR, lane 5). Note decreased binding in the presence of either 100- (100 × GRE-con, lane 3) or 200- (200 × GRE-con, lane 4) fold excess unlabeled consensus sequence or 100-fold GRE-TAT (lane 11, vertical arrow), a known GRE binding sequence. Competition assays with excess unlabeled cd38 putative GRE sequences reveal decreased binding only in the presence of the GRE1 (lane 7, vertical arrow). Note gel supershift in the presence of an anti-GR antibody (shown as anti-GR). Lanes on extreme right show no specific binding of GR to an irrelevant binding site (shown here for CREB, lanes 12 and 13). Representative of 4 assays.