Figure 4

Specific binding of GR tocd38 putative GRE binding sites. Electrophoretic mobility gel shift assays demonstrating binding of nuclear proteins obtained from control or dexamethasone-treated HASM cells to labeled oligonucleotide putative cd38 GRE sites. To confirm specificity of binding, unlabeled oligonucleotide putative cd38 GRE sequences were used as a specific competitor. Dexamethasone induced binding of nuclear proteins to oligonucleotides corresponding to the cd38 putative GRE binding sequences 1, 3 and 4 (labeled GRE1 to GRE4), and decreased binding in the presence of the respective unlabeled oligonucleotide sequences. The binding to GRE3 is weaker compared to the other putative GRE motifs. Note that there is no increase in nuclear protein binding to GRE2 by dexamethasone compared to untreated control.