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Figure 2 | Respiratory Research

Figure 2

From: Regulation of the cd38 promoter in human airway smooth muscle cells by TNF-α and dexamethasone

Figure 2

TNF-α-induced activation of AP-1 in HASM cells. Binding of nuclear proteins to labeled oligonucleotides corresponding to the AP-1 consensus (A) or putative cd38 (B) binding sequences. The specificity of binding was confirmed with excess unlabeled consensus or putative AP-1 oligonucleotide sequences as a specific competitor, and SP1 as a nonspecific competitor. Anti-c-jun or -c-fos antibodies was used for the gel supershift assay. Panel A: TNF-α-induced increased binding to the consensus AP-1 sequence (horizontal arrow) and gel supershift in the presence of an anti-c-Jun antibody (c-Jun). Note decreased binding in the presence of unlabeled consensus AP-1 (AP1) or with mutated AP-1 (AP-1 mut). Panel B: TNF-α-induced increased binding to labeled putative cd38 AP-1 sites 1, 4 and 6 (indicated by arrows and labeled AP1–1, AP1–4 and AP1–6 respectively), with the strongest binding to AP1–4.

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