Figure 5

Frequency of labeled donor cells in lung. The total number of QD-positive donor cells sequestered in the whole lung was calculated by morphometric analysis. The results are shown as the donor cell proportion of total injected cells that were sequestered in the lung. In the whole BM cell transfusion model (A), significantly more donor cells were sequestered in the pneumonia lungs as compared to control (32.6 ± 4.6% vs. 15.3 ± 1.8%, P = 0.007) at 2 hours post-transfusion. After 24 hours, the number of donor cells in lung significantly decreased in both groups, and the difference between the two groups was no longer significant. In the Lin- progenitor cell transfusion model (B), there was a significantly higher number of donor cells sequestered in the pneumonia lungs as compared to control (43.3 ± 8.6% vs. 11.2 ± 3.9%, P = 0.03) at 2 hours post-transfusion. After 24 hours, although the percentage of donor cells decreased in both groups, the ratio of donor cells in the pneumonia group was still significantly higher than control (15.1 ± 3.6% vs. 2.4 ± 1.2%, P = 0.04). Data is shown as mean ± SE; n = 6 (control group for each timepoint) and n = 7 (pneumonia group for each timepoint). *P < 0.05, **P < 0.01.