Figure 5
In vivophagocytosis ofK. pneumoniaeby alveolar macrophages from mice, exposed to ozone. Experimental design was as described in the legend for Figure 1 and the Methods section except that a dose of ~1.2 × 107 CFU/mouse of K. pneumoniae was used, and the mouse lungs were lavaged 1 h post-infection, as described in Methods. Alveolar macrophages were prepared, applied to slides by cytocentrifugation and stained for phagocytosis assessment by light microscopy (see Methods). Results were considered to be significant if p < 0.05 with t-test. (*) significant differences between males and females of the same group (i.e. O3 or FA); (**) significant differences between FA and O3 groups of mice of the same sex. The number of independent experiments was 5 and the number (n) of mice in each group that survived after the procedure is shown in the figure.