Schematic presentation of study design and graph describing tracings and measurements of lung compliance. (A) Experimental protocol. R&Cscan is a program for measuring lung resistance and compliance with the single compartment model. A perturbation of forced oscillation was performed for 4 s (Prime 4, Zrs measurements) and was used in the acute 17-day (OVA'17 and PBS'17 animals) and chronic 98-day protocol (OVA'98 and PBS'98 animals). During A → F, methacholine (MCh) or phosphate buffered saline (PBS) was administrated or nothing was given. MCh or PBS was administrated 20 s after last DI. (B) Tracings of lung compliance (CL) obtained by R&Cscan indicating measurement points for CL (A → F) and ΔCL with and without deep inspirations (DI).