Figure 6
From: Endothelial cell apoptosis in chronically obstructed and reperfused pulmonary artery
A: Proportion of endothelial cells undergoing apoptosis in the distal pulmonary arteries in the sham group and in piglets exposed to ligation for 5 weeks followed by reperfusion for two days, with (AR P+) or without (AR P-) pentoxifylline administration immediately before reperfusion. Typical results from two animals of each group are shown. B: Percent reduction of maximal contraction to phenylephrine produced by acetylcholine stimulation and calcium ionophore stimulation of pulmonary artery rings taken from left lungs 2 days after reperfusion with (AR P+) or without (AR P-) pentoxifylline administration (pentoxifylline+ and pentoxifylline-groups, respectively). Values are means ± SEM. The concentration-response curves were significantly flattened in the pentoxifylline-group and partially restored by administration of pentoxifylline. S: sham group; P-: acute reperfusion without pentoxifylline; P+: acute reperfusion with pentoxifylline. C: Western blot of eNOS protein content (mean ± SEM) 2 days after reperfusion with pentoxifylline (AR P+ group) or without pentoxifylline (AR P- group) compared to the sham group. Typical results from two animals of each group are shown.