Figure 1

Representative cytospin preparation of isolated cells. (A, D, G) CC10 positive airway Clara cells (green) with DAPI counterstain. The same preparations were simultaneously stained for either (B) epithelial cytokeratins, (E) β-tubulin positive ciliated cells, (H) pro-SPC positive alveolar type II cells (all in red). The third panel in each series demonstrates the DAPI nuclear counterstain alone (C,F,I). In panel A, the white arrowhead demonstrates a typical Clara cell, grey arrows indicate an example of a non-epithelial/non-Clara cell and white arrows indicate examples non-Clara, epithelial cells. In D, E and F white arrows indicate the location of 6 ciliated cells, and in G, H and I, white arrows indicate the location of 2 type II cells in the panels. All images at 200×.