Figure 6
GC frass-induced experimental allergic asthma in C57Bl/6 mice. C57Bl/6 mice were sensitized on day 0 and 7 with an intraperitoneal injection of 100 ug/ml PBS or GC frass with alum. On days 14 and 19, an intratracheal inhalation was performed using PBS (40 μl) or GC frass (40 μg/40 ml). On day 22, mice were anesthetized acetylcholine was injected after establishment of a stable airway pressure. A. AHR was measured as airway pressure time index (APTI) in cm-H2O × sec -1 (* p = 0.016). B. Lungs from the mice were excised, and cells dissociated and maintained in a single suspension culture for 3 days in the presence of Con A (10 μg/ml). Supernatants were removed and ELISAs were run for IL-5, IL-13 and IFNγ. These data are represented as cytokine (listed on the x-axis) in ng/ml from PBS or GC frass treated mice (*p = 0.012). C. Serum IgE levels were analyzed by ELISA (*p < 0.001). In all cases the data are expressed as mean ± SEM and represent 6–7 mice per group and statistical significance determined by ANOVA.