Panels A and B: Quantification of bone marrow-derived cells (BMDCs) engraftment in recipient mouse lung by real-time quantitative PCR. A) Relationship between the threshold cycle number and the percentage of male genomic DNA in the samples. The standard curve was generated by using samples containing 100 ng to 0.01 ng of male genomic DNA (losanges). Triangles: DNA from MCTp-treated mice injected with BMDCs 3 days later; squares: DNA from MCTp-treated mice injected with irradiated BMDCs 3 days later; stars : DNA from hypoxia-treated mice injected with BMDCs 3 days later. B) Histogram of the above-reported data showing the percentage of male genomic DNA in lung tissue from female mice treated with MCTp, MCTp followed by BMDCs (black bar), or MCTp followed by irradiated BMDCs (open bar) and hypoxia followed by BMDCs. Values are means ± SEM for 5 animals in each group.