Figure 4

A. Ratio of right ventricle to left ventricle + septum weight [RV/(LV+S)] on day 15 in control untreated mice, hypoxia-exposed mice, hypoxia-exposed mice injected with bone marrow-derived cells (BMDCs), and hypoxia-exposed mice injected with irradiated BMDCs. Values are means ± SEM for 6 animals in each group except control mice (n = 11). B. Right ventricular systolic pressure (RVSP) on day 15 in control untreated mice, hypoxia-exposed mice, hypoxia-exposed mice injected with BMDCs, and hypoxia-exposed mice injected with irradiated BMDCs. Values are means ± SEM for 6 animals in each group. C. Percentage of muscularized intraacinar vessels in lungs on day 15 in control untreated mice, hypoxia-exposed mice, hypoxia-exposed mice injected with BMDCs, and hypoxia-exposed mice injected with irradiated BMDCs. The percentage of pulmonary vessels in each muscularization category was determined by dividing the number of vessels in that category by the total number counted in the same experimental group. Values are means ± SEM for 5 animals in each group except control mice (n = 10). D. Representative panels of proximal artery remodeling. D1) normal artery from a control mouse; D2) hypoxia-exposed mouse with SMC proliferation; D3) hypoxia-exposed mouse injected with BMDCs, with no evidence of recovery of normal arterial morphology; D4) hypoxia-exposed mouse injected with irradiated BMDCs, with no improvement in remodeling or SMC proliferation. Sections were stained with hematoxylin-phloxine-saffron. Original magnification ×500.