SPLUNC1 is not expressed in inflammatory cells in CF or pneumonia. Serial sections of lung tissue were stained as described in the materials and methods section. SPLUNC1 (A) is not found to be expressed in CD68 expressing cells (Macrophages, B) nor in neutrophil elastase expressing cells (Neutrophils, C) in CF. Arrows in A point to the SPLUNC1 negative lumen as well as the positively staining epithelial cell layer. In sections from a case of bacterial pneumonia neither macrophages (E) nor neutrophils (F) appear to stain for SPLUNC1 (D), which is negative in this field.