Figure 6From: Simian virus 40 vectors for pulmonary gene therapyRT-PCR for luciferase mRNA detection: High levels of mRNA were detected in the lungs of 2CLP animals (plasmid pGL3 used as positive control for luciferase). Minimal levels were present in the liver, kidney spleen and none in the heart. Minimal expression in the lungs, kidney, liver, spleen, and heart of T0 and SO animals. GAPDH was used as a house keeping control gene, using PCR primers specific for GAPDH.Back to article page