Figure 5

VP1 detection in type II cells. The cells were double-stained for VP1 and Pro-SP C as described in Materials and Methods. An SO lung is shown at the top row and ARDS lung in the bottom row. VP1 was detected by Cyte5 (red fluorescence, left panels) and Pro-SP C by FITC (green fluorescence, middle panels). The upper and lower right images show co-localization of both stainings inside alveolar type II cells (yellow). Shown at ×100 magnification