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Figure 1 | Respiratory Research

Figure 1

From: Sialic acid receptor detection in the human respiratory tract: evidence for widespread distribution of potential binding sites for human and avian influenza viruses

Figure 1

Effect of different retrieval techniques on lectin binding. Single labelling of paediatric respiratory mucosa by FITC- conjugated Sambucus nigra agglutinin (SNA) (A-F) and Maackia amurensis agglutinin (MAA) (G-J) using different methods of retrieval. No antigen retrieval, (A) and (F), Citrate buffer (B) and (G), EDTA (C) and (H), Pronase (D) and (I), and Trypsin (E) and (J). Stain intensity was graded as strong (++) and a weaker pattern as +. In the absence of unmasking techniques there was minimal to weak (-/+) SNA binding and weak (+) MAA binding in the basal epithelium and epithelial cells of the bronchial mucosa of paediatric tissues. All forms of retrieval enhanced the lectin staining of the surface epithelial cells and mucus containing cells for both SNA and MAA. Examination with dual FITC/Rhodamine filter. Magnification × 200.

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