Figure 3
From: CpG oligonucleotide activates Toll-like receptor 9 and causes lung inflammation in vivo
Pulmonary proinflammatory cytokine gene expression. Time course of pulmonary proinflammatory cytokine gene expression of TNF-α and IL-1β and the house keeping gene L32 following CpG-ODN stimulation in WT and TLR9-D mice. Densitometric analysis of the RNase Protection Assays revealed significant increases of TNF-α-mRNA/L32-mRNA (B) and IL-1β-mRNA/L32-mRNA (C) in WT mice at 1 hr and 2 hrs compared to TLR9-D animals (mean ± SEM; * p < 0.05; AU = arbitrary units).