Table 1 MTT-based cytotoxicity assay of MAPk inhibitors in HAM. HAM were incubated for 24 h with PD98059, SB203580, SP600125 or medium at different concentrations, and cell viability was assessed by using the MTT reduction assay, as described in Methods. Results are mean ± SD of one representative experiment in triplicates (expressed as % of control); *p value < 0.05 (one sample t-test).
Concentration of inhibitors | PD98059 | SB203580 | SP600125 |
|---|---|---|---|
1 μM | 96.2 ± 20.26 | 92.7 ± 14.2 | 92.6 ± 19.5 |
5 μM | 93.4 ± 14 | 85.3 ± 34.6 | 104.9 ± 33.5 |
10 μM | 79.9 ± 14.2 | 104.1 ± 15.7 | 110.1 ± 11 |
25 μM | 122.3 ± 6.9 | 82.8 ± 7.2 | 89.3 ± 9 |
50 μM | 104.6 ± 20.6 | 66.2 ± 10.1* | 65.2 ± 9* |