Figure 7

Sp1 binding activity in nuclear proteins of HAM. HAM were preincubated 1 h or not with inhibitors (50 μM for PD98059, SB203580 and SP600125 or 500 nM for mithramycin) and then stimulated with LPS (1 μg/ml) during 2 hours. Control HAM were HAM cultured with medium alone. Nuclear proteins were extracted as previously described and were then incubated in the binding buffer with specific labelled probe and then subjected to electrophoresis for separation of the complexes.